Features of human monocytic ehrlichiosis laboratory diagnostics

Objective. Using the data obtained in Perm Region as an example, to identify effectiveness of polymerase chain reaction (PCR) for diagnosis Human Monocytic Ehrlichiosis (HME) at different periods from onset disease, and determine role HME structure infections transmitted by ixodic ticks using PCR enzyme-linked immunosorbent assay (ELISA)
 Materials methods. A thorough clinical epidemiological examination 583 patients with acute febrile diseases developed after suction was carried out. To detect E. muris DNA, 1586 whole blood samples were examined disease. For purpose serological verification HME, all ELISA presence immunoglobulins M G against chaffeensis.
 Results. In total, method, ehrlichial DNA detected 76 (4.8 %) 53 patients. Based on two research methods (ELISA PCR) diagnosed 58 (9.9 persons, while 50 (86.2 them, confirmed only PCR. The timing genomic material detection varied 1 days moment greatest (up 69.4 % positive samples) noted us 1st 7th day illness. found form monoinfection 9 (15.5 %), mixed infection 49 (84.5 persons. following revealed: HME+Ixodid tick-borne borreliosis (ITBB) 35 (60.3 HME+ITBB+Human granulocytic anaplasmosis (HGA) 6 (10.3 HME+ITBB+HGA+Tick-borne encephalitis (TBE) 4 (6.9 HME+TBE 2 (3.5 HME+TBE+ITBB %).
 Conclusions. significantly increased number 86.2 cases, most often period disease 15.3 first week disease). laboratory it is advisable combine especially case negative results studies.